Analysis of risk factors of 72 cases with postpartum hemorrhage / 中国基层医药

Objective To explore the risk factors of postpartum hemorrhage .Methods From January 2015 to January 2017,72 cases with postpartum hemorrhage in Rongjun Hospital of Lyuliang were selected in the present study.Logistic regression analysis was used to screen the risk factors of postpartum hemorrhage ,and put forward corre－sponding preventive measures according to different risk factors ,in order to reduce the incidence of postpartum hemor－rhage.Results Age[the incidence rate of postpartum hemorrhage in patients more than 33 years old was 15.63%(5/32)],childbirth way [ the incidence rate of postpartum hemorrhage in cesarean section patients was 28.57%(4/14),gestational age[the incidence rate of postpartum hemorrhage in patients with gestational age ＜41 weeks was 7.41%(4/54)],placenta previa [the incidence rate of postpartum hemorrhage was 23.08%(6/26)],the birth canal injury[the incidence rate of postpartum hemorrhage was 14.29%(5/35)],uterine inertia[the incidence rate of postpartum hemorrhage was 37.93%(22/58)],blood coagulation dysfunction [the incidence rate of postpartum hemorrhage was 8.33%(2/24)] and placenta conglutination [the incidence rate of postpartum hemorrhage was 23.08%(3/13)]had influence on postpartum hemorrhage ,through the study of the statistical analysis of a single risk factor,found that the differences were statistically significant (χ2＝15.125,22.034,15.125,22.034,7.114,25.147, 26.301,8.226,all P＜0.05).Through the analysis of single factor and multiple factors unconditioned logistic regres －sion analysis,established risk factor model and found that cesarean section ,uterine inertia,placenta conglutination conformed to the analysis results (β＝2.868,1.484,1.173,E.(β)＝0.088,0.086,0.175,S.95%CI＝3.499－22.734,2.889－6.834,1.368－3.894),but statistically significant differences were observed among different factors (OR＝16.746,4.0123,2.379,all P＜0.05).Conclusion Age,childbirth way,gestational age,placenta previa, birth canal injury, uterine inertia, blood coagulation dysfunction and placenta adhesion all can affect postpartum hemorrhage,in the process of childbirth pregnant women ,should have the possibility of various factors are positive and effective prevention,ready to related work and measures ,in patients with postpartum should also increase the intensity of nursing,preventing postpartum hemorrhage occurs .

Efficacy of cimetidine combined with acyclovir in treatment of children with varicella / 药物评价研究

Objective To discuss the efficacy ofcimetidine combined with acyclovir in treatment of children with varicella.Methods 80 cases ofvaricella were randomly divided into two groups.The observation group (40 cases) was treated with cimetidine combined with acyclovir treatment.The control group (40 cases) was given the acyclovir.Observe and record the two groups of children with clinical efficacy,fever time,rash scab,itching disappeared time after treatment,before and after treatment during the HAMA score and the treatment of adverse reactions.The clinical curative effect of cimetidine combined with acyclovir in treatment of children with varicella was evaluated.Results The observation group of total effective rate was 87.5％.The control group of total effective rate was 65％.The total effective rate of observation group was higher than the control group,the difference was statistically significant (P ＜ 0.05).After treatment,the fever time,skin rash,scab time and pruritus disappeared in the observation group were shorter than those in the control group,the difference was statistically significant (P ＜ 0.05).Before treatment,there was no statistical difference in HAMA scores between the two groups.After treatment,the scores of HAMA in the two groups were significantly lower than those in the same group before and after treatment (P ＜ 0.05),and the treatment group was significantly lower than the control group,the difference was statistically significant (P ＜ 0.05).There was no significant difference in adverse reactions between the two groups during the treatment.Conclusion Cimetidine combined with acyclovir have a good therapeutic effect on varicella.They can rapidly improve the clinical symptoms of pediatric varicella,reduce anxiety with high safety.It is worthy of clinical use.

Analysis of HIV infection status among outpatients of a general hospital in Yangzhou / 国际检验医学杂志

Objective To understand the distribution characteristics of HIV infection status among the outpatients in this hospi-tal to provide the beneficial supplement for local large-scale survey and the basis especially for the hospital infection control.Meth-ods The HIV screening situation on the outpatients of our hospital from 2007 to 2012 was retrospectively analyzed.Results Dur-ing these 6 years,108 999 specimens were detected,88 cases were positive in the preliminary screening and 81 cases were confirmed positive.The positive rate of anti-HIV antibody was 0.074%.In addition to voluntary counseling and testing,the specimens submit-ted by the outpatient anorectal department and the inpatient hematology department had the higher positive detection rate,which was 1.16% and 0.84% respectively.Conclusion The HIV infection rate in non-infectious disease specialized hospitals shows the increasing trend.The key departments must increase the intensity of the medical staff training,pay attention to early diagnosis of HIV/AIDS,prevent the hospital infection and reduce the risk occurred after the occupation exposure.

Expression of human aspartyl beta-hydroxylase and preparation of its monoclonal antibody / 生物工程学报

We investigated the mechanism of human aspartyl beta-hydroxylase (HAAH) in early diagnosis of tumors. The encoding gene of HAAH was cloned from the hepatic carcinoma by RT-PCR and expressed as a fused protein in the prokaryotic vector pBV-IL1. The expressed HAAH was purified by Ni(2+)-NTA purification column and the purified protein was then used to immunize Balb/c mice. Three hybridoma cell lines (respectively designated H3/E10, E4/F12 and G4/D8) stably expressing the monoclonal antibody specific to HAAH fusion protein were obtained. The specificity and sensitivity of the monoclonal antibody were assessed by indirect enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Finally, the monoclonal antibody expressed by H3/E10 cell line was used to detect the expression of HAAH in several tumor cell lines by indirect immuno-fluorescence, and the specific fluorescence was observed. In conclusion, this study successfully constructed the recombinant prokaryotic vector pBV-IL1-HAAH and prepared HAAH-specific monoclonal antibody for further study of the structure and function of the protein. The result may also lay solid foundation for the research of the molecular mechanism of HAAH in early diagnosis of tumors.

An atomic force microscopy study on the images of para influenza virus under different treatment conditions / 生物医学工程学杂志

Using atomic force microscope (AFM), we investigated the images of Pars influenza virus (PIV) under different treatment conditions and observed the different appearances of the virus and its ultra-microstructure from the exterior to the interior. From the 2D images under transmission electron microscope (TEM), we could see that the surfaces of PIV particles exhibited spherical and band-shaped 'tufts'; from the 3D images under AFM, we could further observe the whole spherical virus particles and their detailed surfaces, which exhibited round and band-shaped 'tufts'. Comparing the images under TEM with those under AFM, we found that the latter could reveal the surface topograph and ultramicrostructure of viruses more truly than did the former. The samples of viruses were treated by Tritonx-100, the lipid envelopes of virions were partly or completely resolved, and then most of their capsids were exposed. We could observe the different appearances of the virions under AFM, the lipid envelopes of which were gradually removed. The samples of viruses were also treated by SDS, and the RNA was released from the virions. From the AFM images, we could see the structure of the RNA. It was thus clear that AFM could be used to investigate the different appearances and ultramicrostructure of viruses rapidly and efficiently.

Construction of a recombinant adenovirus carrying the Hantaan virus glycoprotein G2 gene and its expression in Hela cells / 生物工程学报

We constructed the recombinant adenovirus expressing the glycoprotein G2 of Hantaan virus. Firstly we obtained coding gene fragment of G2 by PCR, and subsequently inserted the gene of interest into the Adenoviral pShuttle vector pAd5-CMV. Then we co-transfected the recombinant pShuttle vector and adenovirus skeleton plasmid into HEK293 cells by Calcium phosphate precipitation method. After the recombinant adenovirus were packaged and amplified in HEK293 cells, we observed the expression of reporter gene eGFP by fluorescent microscopy, and we obtained the recombinant adenovirus containing Hantaan virus glycoprotein G2. The recombinant adenoviruses were used to infect Hela cells and the expressed protein was detected by Indirect Immuno-fluorescence and Western blotting. The construct was confirmed at several levels: first restriction enzyme analysis demonstrated that the recombinant adenovirus vector was constructed correctly, second RT-PCR showed that the G2 gene could transcribe correctly in Hela cells. Then Fluorescent microscopy proved the expression of eGFP in the infected Hela cells. Finally, Indirect Immune-fluorescence and Western-blot confirmed the expression of interested protein identified by anti-G2 monoclonal antibody. In conclusions, this study successfully constructed the recombinant adenovirus containing Hantaan virus envelope glycoprotein G2, meanwhile obtained the G2 protein, it may lay solid foundation for the structure study of G2 protein and the new vaccine of Hantaan virus.

Purification and identification of recombinant nuclear protein of Hantaan virus / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To purify recombinant nuclear protein of Hantaan virus.</p><p><b>METHODS</b>The recombinant plasmid was transformed into E.coli and induced by IPTG. The expressed protein is a fusion protein with GST and existed in inclusion bodies. The inclusion bodies were processed through denaturation and renaturation and were purified with Glutathione Sepharose 4B affinity chromatography column. Then the purified fusion protein and nuclear protein were examined by sandwich ELISA and Western blot.</p><p><b>RESULTS</b>The expressed fusion protein was separated from the mixture proteins by the first affinity chromatography. GST was cut from the purified fusion protein with thrombin. The nuclear protein was separated from GST by the second affinity chromatography. The crossed peak represents nuclear protein and the elute peak represents GST. The purified fusion protein and nuclear protein were single band by SDS-PAGE. Both of them had available antigen activity.</p><p><b>CONCLUSIONS</b>Purification of nuclear protein of Hantaan virus with Glutathione Sepharose 4B affinity chromatography is an effective method.</p>

Expression of hepatitis C virus H strain envelope glycoprotein in insect cells / 细胞与分子免疫学杂志

Aim To express hepatitis C virus glycoprotein E (gE) deleting carboxy-terminal 31 amino acids, and detect anti-E antibody in HCV patients using expressed gE. Methods E gene derived from HCV H strain was inserted into baculovirus transfer vector containing a polyhedrin promotor. The recombinant plasmid was cotransfected into insect cell sf9 with a viral expression vector. The expression of gE was analyzed with Western blot, and the cells were used for dectecting antibodies against E1 and E2 in 35 hepatitis C patients by indirect immunofluorescence. Results A series of proteins with different relative molecular masses(Mr) could be detected by Western blot. Results from indirect immunofluorescence staining showed and only 4 patients were anti-E antibody positive gE was located in cytoplasm. Conclusion HCV gE is expressed successfully in insect cells, the study lay the foundation for further developing HCV vaccine.

Study on antitumor effect by gene immunization of the chimeric HBV and HCV / 中国免疫学杂志

Objective:To observe the specific cellular immune response and the protection against P815 mastovytoma cells(H 2 d) stable expressing HBV surface antigen and HCV core antigen after the immunized mice(H 2 d) with plasmids SpcDNA3.1,CpcDNA3.1 and chimeric plasmid CSpcDNA3.1.Methods:After subcutaneous immunization with the three plasmids SpcDNA3.1,CpcDNA3.1 and CSpcDNA3.1 respecitively 3 w,the mice were inoculated with the transfected P815 tumor cells.The tumors size and the survival rate were measured.CTL assay was detected with LDH methods.Results:The chimeric plasmid CSpcDNA3.1 could inhibit the tumor growth,prolong the survival period and improve the survivial rate evidently.The splencytes from immunized mice showed strong CTL activity to CSpcDNA3.1 transfected P815 tumor cells.Conclusion:It was suggestd that specific antitumor cellular immunity could be induced by immunization with the chimeric plasmid CSpcDNA3.1 that contain chimeric HBV and HCV gene.

Association between Air Pollutants Exposure during Pregnancy and Preterm Delivery in Taiyuan / 环境与健康杂志

Objective To investigate the relationship between air pollutant exposure and preterm delivery (PTD) by Logistic regression model, as the effect of air pollutant exposure to PTD made great concern today. Methods The present study consisted of 31 145 qualified birth cases, 80.09% of the total amount, from 1 Nov, 2005 to 1 Aug, 2007. The covariant items included maternal age, nation, education, residence, income, occupation, occupation exposure, parent-drinking, father-smoking, heating-type, folic acid supplement, season of pregnancy, prenatal health care, etc. The Logistic regression analysis was employed to control the confounding factors mentioned above, then the pollutant was introduced step by step to build the single, double and triple pollutant Logistic regression model to examine the association between three pollutants (SO2, NO2, and PM10) and PTD during the first and third trimesters of pregnancy. The air monitoring data (daily average of SO2, NO2, and PM10) were collected from Taiyuan Environmental Monitoring Station. Results In the first trimester, a quartile increase of NO2 exposure caused the risk for PTD increased by 22.7%. As for the effect of PM10, no significant differences were seen. In the third trimester, a quartile increase of NO2 exposure caused the risk for PTD increased by 19.1%. Conclusion The NO2 exposure during the pregnancy can increase the risk of PTD. The susceptible exposure-window choice is critical for the further study in this field.